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KMID : 0545120090190010011
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 1 p.11 ~ p.16
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Functional Analysis of MCNA;a Gene Encoding a Catalytic Subunit of Calcineurin;in the Rice Blast Fungus Magnaporthe oryzae
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Lee Yong-Hwan
Choi Jin-Hee
Lee Yong-Hwan
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Abstract
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Magnaporthe oryzae, the causal agent of rice blast, forms a specialized infection structure, called an appressorium, which is crucial for penetration and infection of the host plant. Pharmacological data suggest that calcium/calmodulindependent signaling is involved in appressorium formation in this fungus. To understand the role of the calcium/ calmodulin-activated protein phosphatase on appressorium formation at the molecular level, MCNA, a gene encoding the catalytic subunit of calcineurin, was functionally characterized in M. oryzae. Transformants expressing sense/antisense RNA of MCNA exhibited significant reductions in mycelial growth, conidiation, appressorium formation, and pathogenicity. cDNA of MCNA functionally complemented a calcineurin disruptant strain (cmp1::LEU2 cmp2::HIS3) of Saccharomyces cerevisiae. These data suggest that calcineurin A plays important roles in signal transduction pathways involved in the infection-related morphogenesis and pathogenicity of M. oryzae.
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KEYWORD
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Calcineurin, Magnaporthe oryzae, calmodulin,
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